(Click on a character's name for a full description.)
| Name | Gender | Vocal Part | Age Range | Role Type |
| "Mama" Morton | female | alto | 27 – 40 | lead |
| Aaron | male | cameo | ||
| Amos Hart | male | baritone | 24 – 32 | lead |
| Annie | female | cameo | ||
| Billy Flynn | male | baritone | 30 – 45 | lead |
| Chorus | female | 18 – 55 | chorus | |
| Court Clerk | male | cameo | ||
| Fred Casely | male | cameo | ||
| Go-To-Hell-Kitty | female | cameo | ||
| Harry | male | cameo | ||
| Hunyak | male | cameo | ||
| Judge | male | cameo | ||
| June | female | cameo | ||
| Martin Harrison | male | cameo | ||
| Mary Sunshine | N/A | alto | 27 – 50 | supporting |
| Mona | female | cameo | ||
| Roxie Hart | female | mezzo | 20 – 28 | lead |
| Sergeant Fogarty | male | cameo | ||
| Velma Kelly | female | alto | 23 – 30 | lead |
